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Plant Kinases - Methods and Protocols
- 1.0369271 - ÚEB 2012 RIV US eng M - Monography Chapter
Spíchal, Lukáš
Bacterial assay to study plant sensor histidine kinases.
Plant Kinases - Methods and Protocols. New York City: Humana Press, 2011 - (Dissmeyer, N.; Schnittger, A.), s. 139-147. Methods in Molecular Biology, 779. ISBN 978-1-61779-263-2
R&D Projects: GA ČR GA301/08/1649; GA MŠMT(CZ) LC06034
Institutional research plan: CEZ:AV0Z50380511
Keywords : Cytokinins * histidine kinases * CRE1/AHK4
Subject RIV: EF - Botanics
http://www.ncbi.nlm.nih.gov/pubmed/21837564 http://arl-repository.lib.cas.cz/uloziste_av/UEB-Q/cav_un_epca-0369271_01.pdf
Cytokinins, like other phytohormones, act in plants as signaling molecules at very low concentrations. The system that mediates between their chemical recognition and the responses that they induce requires a hormone receptor that, together with down-stream located elements, forms a signaling network, converting the signal into a specific response. Identification of the cytokinin-binding histidine kinases CRE1/AHK4, AHK3, and AHK2 as cytokinin receptors in Arabidopsis was an important milestone in the elucidation of cytokinin signal transduction pathways. Their molecular characterization through the use of transgenic E. coli strains revealed that a variety of cytokinin compounds may have signaling functions, but only with specific receptors. This indicates that differential ligand specificities of the receptors may be a mechanism to fine-tune the various cytokinin responses.
Permanent Link: http://hdl.handle.net/11104/0203377
Number of the records: 1