Characterization of microorganisms using Raman tweezers

0368396 - ÚPT 2012 RIV US eng C - Conference Paper (international conference)
Samek, Ota - Pilát, Zdeněk - Jonáš, Alexandr - Zemánek, Pavel - Šerý, Mojmír - Ježek, Jan - Bernatová, S. - Nedbal, L. - Trtílek, M.
Characterization of microorganisms using Raman tweezers.
Optical Trapping and Optical Micromanipulation VIII (Proceedings of SPIE Vol. 8097). Bellingham: SPIE, 2011, 80970F:1-7. ISBN 978-0-8194-8707-0.
[Conference on Optical Trapping and Optical Micromanipulation VIII. San Diego (US), 21.08.2011-25.08.2011]
R&D Projects: GA MŠMT ED0017/01/01; GA ČR GAP205/11/1687; GA MPO FR-TI1/433
Grant - others:EC(XE) PERG 06-GA-2009-256526
Institutional research plan: CEZ:AV0Z20650511
Keywords : Raman spectroscopy * bacteria * algal cells * lipids * iodine value
Subject RIV: BH - Optics, Masers, Lasers

The ability to identify and characterize microorganisms (algae, bacteria, eukaryotic cells) from minute sample volumes in a rapid and reliable way is the crucial first step in their classification and characterization. In the light of this challenge related to microorganisms exploitation Raman spectroscopy can be used as a powerful tool for chemical analysis. Raman spectroscopy can elucidate fundamental questions about the metabolic processes and intercellular variability on a single cell level. Moreover, Raman spectroscopy can be combined with optical tweezers and with microfluidic chips to measure nutrient dynamics and metabolism in vivo, in real-time, and label free. We demonstrate the feasibility to employ Raman spectroscopy-based sensor to sort microorganisms (bacteria, algae) according to the Raman spectra. It is now quite feasible to sort algal cells according to the degree of unsaturation (iodine value) in lipid storage bodies.
Permanent Link: http://hdl.handle.net/11104/0202753