Porcine anti-polyspermy defense involves sperm surface p

0367655 - BTÚ 2012 AU eng A - Abstract
Jonáková, Věra - Postlerová, Pavla - Yi, Y. - Sutovsky, P. - Pěknicová, Jana
Porcine anti-polyspermy defense involves sperm surface p.
The book of abstracts of the World Congress on Reproductive Biology. Cairns: Society for Reproductive biology, 2011 - (Russell, D.; Okuda, K.; FitzHarris, G.). s. 124-124
[The Second Worl Congress on Reproductive Biology. 09.10.2011-12.10.2011, Cairns]
R&D Projects: GA ČR(CZ) GA303/09/1285; GA MŠMT(CZ) 1M06011; GA MZd(CZ) NS10009-4/2008
Institutional research plan: CEZ:AV0Z50520701
Keywords : spermadhesins * ubiquitin-proteasome * zona pellucida * polyspermy
Subject RIV: CE - Biochemistry

We found that the activity and turnover of the porcine AQN1 protein (and thus the efficiency of the sperm-ovum recognition process) may be controlled by elements of the sperm surface bound ubiquitin-proteasome pathway (UPP), by ubiquitin C-terminal hydrolase UCHL3, and by subunit PSMD8 of the 19S proteasomal regulatory complex. Ubiquitinated isoforms of AQN1 were detected in boar sperm extracts. The UCH-inhibitor ubiquitin–aldehyde, and the antibodies against UCHL1 or PSMD8 increased the rate of sperm-ZP penetration and polyspermy during porcine IVF. In contrast, the addition of recombinant UCHL3, into fertilization medium reduced polyspermy rates. These results are of significance for the amelioration of high polyspermy (~50%) during porcine IVF for commercial embryo transfer, e.g. by the modulation of the UCHL and/or PSMD8 activity.
Permanent Link: http://hdl.handle.net/11104/0202248