Rapid identification of microorganisms using electromigration techniques and MALDI-TOF mass spectrometry

0366989 - ÚIACH 2012 CZ eng A - Abstract
Šalplachta, Jiří - Horká, Marie - Kubesová, Anna - Moravcová, Dana - Růžička, F. - Horký, J.
Rapid identification of microorganisms using electromigration techniques and MALDI-TOF mass spectrometry.
CECE 2011. Brno: Ústav analytické chemie AV ČR v. v. i., 2011 - (Foret, F.). s. 62. ISBN 978-80-904959-0-6.
[International Interdisciplinary Meeting on Bioanalysis /8./. 03.11.2011-04.11.2011, Brno]
R&D Projects: GA MV VG20112015021
Institutional research plan: CEZ:AV0Z40310501
Keywords : microorganisms * MALDI-TOF MS * Candida psilosis
Subject RIV: CB - Analytical Chemistry, Separation

Several different strains of Monilinia and Candida spp. were subjected to analysis by three well-established analytical techniques – CIEF, IEF and MALDI-TOF MS. Our results have revealed that that isoelectric points, migration velocities or the protein patterns can be used as the identification markers. The proposed methods are suitable for rapid and reliable identification of both Monilinia and Candida „psilosis“ spp. in a common laboratory, which could be used for correct disease diagnosis and subsequent successful treatment of an infection.
Permanent Link: http://hdl.handle.net/11104/0201793