Enzymatic synthesis of dimeric glycomimetic ligands of NK cell activation receptors

0366522 - MBÚ 2012 RIV NL eng J - Journal Article
Drozdová, Anna - Bojarová, Pavla - Křenek, Karel - Weignerová, Lenka - Hensen, B. - Elling, L. - Christensen, H. - Jensen, H.H. - Pelantová, Helena - Kuzma, Marek - Bezouška, Karel - Krupová, Monika - Adámek, David - Slámová, Kristýna - Křen, Vladimír
Enzymatic synthesis of dimeric glycomimetic ligands of NK cell activation receptors.
Carbohydrate Research. Roč. 346, č. 12 (2011), s. 1599-1609. ISSN 0008-6215. E-ISSN 1873-426X
R&D Projects: GA ČR GP203/09/P024; GA ČR GD305/09/H008; GA ČR GA303/09/0477
Institutional research plan: CEZ:AV0Z50200510
Keywords : beta-N-Acetylhexosaminidase * alactosyltransferase * Enzymatic glycosylation
Subject RIV: CE - Biochemistry
Impact factor: 2.332, year: 2011

This work reveals new structural relationships in the complex process of the interaction between activation receptors of natural killer cells (rat NKR-P1, human CD69) and novel bivalent carbohydrate glycomimetics. The length, glycosylation pattern and linker structure of receptor ligands were examined with respect to their ability to precipitate the receptor protein from solution, which simulates the in vivo process of receptor aggregation during NK cell activation. It was found that di-LacdiNAc triazole compounds show optimal performance, reaching up to 100 percent precipitation of the present protein receptors, and achieving high immunostimulatory activities without any tendency to trigger activation-induced apoptosis. In the synthesis of the compounds tested, two enzymatic approaches were applied
Permanent Link: http://hdl.handle.net/11104/0201491