Sample preparation in separation of the extracellular chitinolytic enzymes of the human intestinal bacterium Clostridium paraputrificum J4 from the culture fluids

0360819 - ÚMCH 2012 RIV NL eng J - Journal Article
Tishchenko, Galina - Šimůnek, Jiří - Bartoňová, Hana - Dušková, Jarmila - Dohnálek, Jan - Ponomareva, Evgenia - Tennikova, T.
Sample preparation in separation of the extracellular chitinolytic enzymes of the human intestinal bacterium Clostridium paraputrificum J4 from the culture fluids.
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 879, č. 22 (2011), s. 2175-2178. ISSN 1570-0232. E-ISSN 1873-376X
R&D Projects: GA ČR GA310/09/1407; GA ČR GA525/05/2584
Institutional research plan: CEZ:AV0Z40500505; CEZ:AV0Z50450515
Keywords : sample preparation * culture fluids C. paraputrificum J4 * chitinases
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.888, year: 2011

Membrane ultrafiltration (UF) was used in sample preparation of the culture fluids of the human intestinal bacterium Clostridium paraputrificum strain J4 containing seven extracellular chitinolytic isoenzymes (38–90 kDa). The subsequent filtration of the bacteria-free supernatants was carried out through Millipore membranes with cut-off 100 and 30 kDa for separation of undigested components of the culture medium and bacterial metabolites with molecular weight higher and lower than that of the target enzymes. On the basis of the obtained experimental results, the sample preparation procedure of the culture fluids of C. paraputrificum J4 was established to be used further in chromatographic separations of the chitinolytic enzymes.
Permanent Link: http://hdl.handle.net/11104/0198281