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Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber
- 1.0360791 - MBÚ 2012 RIV JP eng J - Journal Article
Siřišťová, L. - Luhový, R. - Sigler, Karel - Řezanka, Tomáš
Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber.
Extremophiles. Roč. 15, č. 3 (2011), 423-429. ISSN 1431-0651. E-ISSN 1433-4909
Institutional research plan: CEZ:AV0Z50200510
Keywords : Thermophilic bacteria * Geobacillus * Meiothermus
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.941, year: 2011
Two thermophilic strains belonging to Geobacillus stearothermophilus and Meiothermus ruber, which naturally do not synthesize omega-alicyclic fatty acids (omega-FAs) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. Gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize omega-FAs when cultivated with the appropriate precursor. The incorporation of cyclic acids influenced the whole FA composition as well as membrane fluidity. Membrane fluidity of intact cells was studied by measuring the fluorescence polarisation of the probe l,6-diphenyl-1,3,5-hexatriene incorporated into membrane lipid bilayers. Cytoplasmic membrane became more fluid with increasing content of omega-FAs. This is caused by considerable changes in lipid packing within the membrane induced by the presence of omega-FAs not found in the natural environment of Geobacillus and Meiothermus strains
Permanent Link: http://hdl.handle.net/11104/0198258
Number of the records: 1