Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing

0359988 - ÚOCHB 2012 RIV US eng J - Journal Article
Koval, Dušan - Kašička, Václav - Cottet, H.
Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing.
Analytical Biochemistry. Roč. 413, č. 1 (2011), s. 8-15. ISSN 0003-2697. E-ISSN 1096-0309
R&D Projects: GA ČR GP203/09/P485; GA ČR(CZ) GA203/08/1428
Institutional research plan: CEZ:AV0Z40550506
Keywords : capillary zone electrophoresis * capillary isoelectric focusing * glycated hemoglobin HbA1c
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.996, year: 2011

Two capillary electrophoretic methods were developed and evaluated for measurement of glycated hemoglobin HbA1c. Firstly, a capillary zone electrophoresis analysis is performed with a sodium tetraborate buffer of pH 9.3 as background electrolyte in a neutrally coated capillary. HbA1c is separated from HbA0 due to specific interactions of borate anions with the cis-diol pattern in the saccharide moiety of glycohemoglobin. Secondly, a capillary isoelectric focusing method, which exploits a difference in pI values of HbA0 and HbA1c, is performed with Servalyt pH 6-8 or alternatively with Biolyte pH 6-8 carrier ampholytes spiked with a narrow pH cut of pH 7.2 prepared by preparative fractionation of Servalyt pH 4-9 carrier ampholytes.
Permanent Link: http://hdl.handle.net/11104/0197650