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Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53
- 1.0358220 - ÚFE 2011 RIV DE eng J - Journal Article
Šípová, Hana - Špringer, Tomáš - Homola, Jiří
Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53.
Analytical and Bioanalytical Chemistry. Roč. 399, č. 7 (2011), s. 2343-2350. ISSN 1618-2642. E-ISSN 1618-2650
R&D Projects: GA AV ČR KAN200670701; GA MŠMT OC09058; GA ČR GA202/09/0193
Institutional research plan: CEZ:AV0Z20670512
Keywords : Surface Plasmon Resonance * point mutation * amplification
Subject RIV: JB - Sensors, Measurment, Regulation
Impact factor: 3.778, year: 2011
This paper reports an approach to detection of single nucleotide polymorphism based on special amplification assay and surface plasmon resonance (SPR) biosensor technology. In this assay, a part of the target DNA is recognized by a probe coupled with streptavidin-oligonucleotide (SON) complexes ex-situ. The SON-target complex is then detected with the SPR sensor with high specificity and sensitivity. The assay is applied to detection of short analogue of TP53 at levels down to 40 pM.
Permanent Link: http://hdl.handle.net/11104/0196317
Number of the records: 1