0357273 - MBÚ 2011 RIV SK eng O - Others
Štěpánek, Václav - Kotík, Michael - Marešová, Helena - Valešová, Renata - Grulich, Michal
Novel prokaryotic epoxide hydrolase and Baeyer-Villiger monooxygenase genes from metagenome.
KONGRES ČESKOSLOVENSKEJ SPOLOČNOSTI MIKROBIOLOGICKEJ /25./. Bratislava: Československá spoločnosť mikrobiologická, 2010. s. 57-57. ISBN 970-80-970477-8-8
Institutional research plan: CEZ:AV0Z50200510
Keywords : epoxide hydrolases * environmental DNA
Subject RIV: EE - Microbiology, Virology

Three genes encoding functional epoxide hydrolases and four hypothetical Baeyer-Villiger monooxygenase genes were obtained directly from environmental DNA using PCR with consensus-degenerate hybrid oligonucleotide primers followed by semi-specific genome-walking PCR. Retrieved complete structural genes showed high GplusC content (mostly over 61percent) and a database search revealed that maximum percentage identity of these novel proteins to known or putative epoxide hydrolases and monooxygenases varied from 41 to 73, and from 47 to 69, respectively
Permanent Link: http://hdl.handle.net/11104/0195591