Determination of butoxyacetic acid (biomarker of ethylene glycol monobutyl ether exposure) in human urine candidate reference material

0356676 - ÚJF 2011 RIV DE eng J - Journal Article
Sperlingova, I. - Dabrowská, L. - Stránský, V. - Duskova, S. - Kučera, Jan - Tvrdíková, M. - Tichý, M.
Determination of butoxyacetic acid (biomarker of ethylene glycol monobutyl ether exposure) in human urine candidate reference material.
Analytical and Bioanalytical Chemistry. Roč. 397, č. 2 (2010), s. 433-438. ISSN 1618-2642. E-ISSN 1618-2650.
[12th International Symposium on Biological and Environmental Reference Materials Keble Coll. Oxford, 07.07.2009-10.07.2009]
Institutional research plan: CEZ:AV0Z10480505
Keywords : Candidate reference material * Ethylene glycol monobutyl ether * Metabolite
Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders
Impact factor: 3.841, year: 2010

Ethylene glycol monobutyl ether (EGBE), an industrial solvent, is absorbed by the body not only by inhalation but also by dermal absorption (liquid or vapour). EGBE is metabolized to butoxyacetic acid (BAA). Pooled freeze-dried urine candidate reference material (RM) was prepared from urine obtained from persons occupationally exposed to EGBE. This material has the advantage of containing butoxyacetic acid in both the free and conjugated (glutamine and glycine) forms, as found in native urine. In all GC method modifications used, acid hydrolysis was used to release BAA from its conjugated form. The amount of butoxyacetic acid in homogeneity and stability testing was measured by GC after derivatisation with N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide. Detection was by MS in EI mode, in the authors' laboratory. For interlaboratory comparison of the reference material GC methods with MS, FID, and ECD were used.
Permanent Link: http://hdl.handle.net/11104/0195136