Genetically modified Candida glabrata strains lacking plasma membrane efflux systems for potassium and podium cations

0355652 - FGÚ 2011 RIV CZ eng L - Prototype, f. module
Krauke, Yannick - Sychrová, Hana
Genetically modified Candida glabrata strains lacking plasma membrane efflux systems for potassium and podium cations.
Internal code: C. glabrata ena1 a cnh1 ; 2010
Technical parameters: Mutantní kmeny C. glabrata s vysokou citlivostí k solím alkalických kovů vhodné studium vlivu transportérů na virulenci a pro screening nových látek s antifungálním účinkem
Economic parameters: náklady v 300 000,- Kč, licenční poplatek 50 000,- Kč
R&D Projects: GA MŠMT(CZ) LC531
Institutional research plan: CEZ:AV0Z50110509
Keywords : Candia glabrata * salt-sensitive mutant
Subject RIV: EE - Microbiology, Virology

C. glabrata cnh1 and ena1 mutant strains lacking the alkali-metal-cation exporters encoded by ENA1 and CNH1 genes have been constructed. All genes have been substituted by the Nourseothricin cassette. Finally, the cassettes were excised using a recombinase and leaving a single FRT site in the loci. Unique GMO strains in the C. glabrata background have been created to study the role of alkali-metal-cation exporters in yeast pathogenicity and virulence
Permanent Link: http://hdl.handle.net/11104/0194371