Treatment of ocular surface injuries by limbal and mesenchymal stem cells growing on nanofiber scaffolds

0355008 - ÚMG 2011 RIV US eng J - Journal Article
Zajícová, Alena - Pokorná, Kateřina - Lenčová, Anna - Krulová, M. - Svobodová, E. - Kubinová, Šárka - Syková, Eva - Přádný, Martin - Michálek, Jiří - Svobodová, J. - Munzarová, M. - Holáň, Vladimír
Treatment of ocular surface injuries by limbal and mesenchymal stem cells growing on nanofiber scaffolds.
Cell Transplantation. Roč. 19, č. 10 (2010), s. 1281-1290. ISSN 0963-6897. E-ISSN 1555-3892
R&D Projects: GA AV ČR KAN200520804; GA ČR GD310/08/H077; GA MŠMT 1M0506
Institutional research plan: CEZ:AV0Z50520514; CEZ:AV0Z50200510; CEZ:AV0Z40500505; CEZ:AV0Z50390703
Keywords : nanofibers * stem cells * treatment of injuries
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 6.204, year: 2010

The paper describes the use of novel nanofiber scaffolds fabricated from polyamid P6/12 for the growth of limbal stem cells (LSC) and mesechymal stem cellls (MSC). The cells grew on nanofibers comparable well as on plastic surfaces. The cells growing on these nanofibers were transferred on the damaged ocular surface in experimental mouse nodel where their trafic from the nanofibers onto ocular surface was monitored. The transferred cells supported a healing process after ocular injury and significantly inhibited a local inflammatory reaction. The results thus showed that LSC and MSC growing on nanofiber scaffolds can be successfully used for the treatment of ocular surface injuries and limbal stem cell deficiencies.
Permanent Link: http://hdl.handle.net/11104/0193872