Lateral Brightness Correction in Confocal Microscopy Images Using Mathematical Morphology Filters

0354159 - FGÚ 2011 RIV US eng J - Journal Article
Michálek, Jan - Čapek, M. - Mao, X. W. - Kubínová, Lucie
Lateral Brightness Correction in Confocal Microscopy Images Using Mathematical Morphology Filters.
Microscopy and Microanalysis. Roč. 16, Suppl.2 (2010), s. 758-759. ISSN 1431-9276. E-ISSN 1435-8115.
[Microscopy and Microanalysis 2010. Portland, 01.08.2010-05.08.2010]
R&D Projects: GA MŠMT(CZ) LC06063; GA MŠMT(CZ) ME09010; GA ČR(CZ) GA102/08/0691; GA ČR(CZ) GA304/09/0733
Institutional research plan: CEZ:AV0Z50110509
Keywords : Lipschitz cover * lateral intensity correction * confocal microscopy
Subject RIV: JD - Computer Applications, Robotics
Impact factor: 2.179, year: 2010

We present a fast correction method based on estimating a spatially variable gain which models the lateral brightness distortion, and multiplying acquired CLSM images by the inverse of the estimated gain. The estimation is done from the CLSM image itself, not from some kind of a reference image. The proposed approach exploits two types of mathematical morphology operators: the median filter and the upper Lipschitz cover
Permanent Link: http://hdl.handle.net/11104/0193220