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The expression of NADPH oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line A549
- 1.0353772 - BFÚ 2011 RIV CZ eng J - Journal Article
Kolářová, Hana - Binó, Lucia - Pejchalová, Kateřina - Kubala, Lukáš
The expression of NADPH oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line A549.
Folia Biologica. Roč. 56, č. 5 (2010), s. 211-217. ISSN 0015-5500. E-ISSN 0015-5500
R&D Projects: GA ČR(CZ) GA524/06/1197; GA ČR(CZ) GA524/08/1753
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : lung epithelial cells * reactive oxygen species * NADPH oxidase
Subject RIV: BO - Biophysics
Impact factor: 0.729, year: 2010
Human lung epithelial cell line A549 was found to significantly express NOX1, NOX2, DUOX1 and DUOX2 and ROS production was detected with fluorometric probes 2,7-dichlorofluorescein- diacetate, dihydroethidium, and amplex red or colorimetric probe nitrobluetetrazolium. These results revealed differences among methods used for ROS formation measurement by cell line A549 and highlighted the sensitivity of fluorometric determination for this purpose.
Permanent Link: http://hdl.handle.net/11104/0192924
Number of the records: 1