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Reduction of heteroduplex formation in PCR amplification
- 1.0350890 - BFÚ 2011 RIV NL eng J - Journal Article
Michu, Elleni - Mráčková, Martina - Vyskot, Boris - Žlůvová, Jitka
Reduction of heteroduplex formation in PCR amplification.
Biologia Plantarum. Roč. 54, č. 1 (2010), s. 173-176. ISSN 0006-3134. E-ISSN 1573-8264
R&D Projects: GA AV ČR(CZ) KJB600040801; GA ČR(CZ) GD204/09/H002; GA AV ČR(CZ) IAA600040801; GA MŠMT(CZ) LC06004
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : polymerase chain reaction * reconditioning PCR * mixed-template PCR
Subject RIV: BO - Biophysics
Impact factor: 1.582, year: 2010
We detected that the heteroduplex to homoduplex ratio can be decreased by the addition of Taq polymerase and by a decrease in the number of PCR cycles.
Permanent Link: http://hdl.handle.net/11104/0190768
Number of the records: 1