A Gene in the Process of Endosymbiotic Transfer

0350376 - BC 2011 RIV US eng J - Journal Article
Jiroutová, Kateřina - Kořený, Luděk - Bowler, Ch. - Oborník, Miroslav
A Gene in the Process of Endosymbiotic Transfer.
PLoS ONE. Roč. 5, č. 10 (2010), e13234. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA AV ČR IAA601410907
Institutional research plan: CEZ:AV0Z60220518
Keywords : MINO-ACID REPLACEMENT * DIATOM THALASSIOSIRA-PSEUDONANA * PHAEODACTYLUM-TRICORNUTUM * PHOTOSYSTEM-II * NUCLEOTIDE-SEQUENCE
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.411, year: 2010

We show here that both versions of the psb28 gene in T. pseudonana are transcribed. We also provide experimental evidence for successful targeting of the nuPsb28 fused with EYFP to the diatom complex plastid. Extensive phylogenetic analyses demonstrate that nucleotide composition of the analyzed genes deeply influences the tree topology and that appropriate methods designed to deal with a compositional bias of the sequences and the long branch attraction artefact (LBA) need to be used to overcome this obstacle. We propose that nuclear psb28 in T. pseudonana is a duplicate of a plastid localized version, and that it has been transferred from its endosymbiont.
Permanent Link: http://hdl.handle.net/11104/0190393