Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study

0350303 - ÚFE 2011 RIV GB eng J - Journal Article
Špringer, Tomáš - Šípová, Hana - Vaisocherová, Hana - Štepánek, J. - Homola, Jiří
Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study.
Nucleic Acids Research. Roč. 38, č. 20 (2010), s. 7343-7351. ISSN 0305-1048. E-ISSN 1362-4962
R&D Projects: GA AV ČR KAN200670701
Grant - others:Univerzita Karlova(CZ) SVV-2010-261 304
Institutional research plan: CEZ:AV0Z20670512
Keywords : oligonucleotide * monovalent and divalent cations * surface plasmon resonance
Subject RIV: BO - Biophysics
Impact factor: 7.836, year: 2010

We investigated the simultaneous effect of monovalent and divalent cations on hybridization of DNA targets to DNA probes immobilized on the surface of a surface plasmon resonance sensor. Our results demonstrated that divalent magnesium is found to be much more efficient in duplex stabilization than monovalent sodium. This trend is opposite to that established for oligonucleotides in a solution. Moreover, the selected saline composition of buffer improved the discrimination of complementary and point mismatched DNA targets.
Permanent Link: http://hdl.handle.net/11104/0190338