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Molecular detection of Entoloma spp. associated with roots of rosaceous woody plants

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    0350285 - MBÚ 2011 RIV DE eng J - Journal Article
    Gryndler, Milan - Egertová, Z. - Soukupová, Lucie - Gryndlerová, Hana - Borovička, Jan - Hršelová, Hana
    Molecular detection of Entoloma spp. associated with roots of rosaceous woody plants.
    Mycological Progress. Roč. 9, č. 1 (2010), s. 27-36. ISSN 1617-416X. E-ISSN 1861-8952
    R&D Projects: GA ČR GA526/06/0540
    Institutional research plan: CEZ:AV0Z30130516; CEZ:AV0Z50200510
    Keywords : Fruit trees * Cellobiose * Inoculation
    Subject RIV: EE - Microbiology, Virology
    Impact factor: 1.266, year: 2010

    Seventeen isolates of Entoloma clypeatum, a fungus associated with rosaceous woody plants and suspected to act as a root pathogen, were obtained from fruit-bodies collected at 7 localities of the Czech Republic. Culture conditions and sensitivity to fungicides have been studied. A PCR primer directed to LSU rRNA gene specific to Entoloma associated with rosaceous plants has been designed. This primer can be used to detect the fungus in cultivated rosaceous fruit trees. The method is robust towards the false positive detection caused by nonspecific amplification of DNA of other soil microorganisms
    Permanent Link: http://hdl.handle.net/11104/0190322

     
     
Number of the records: 1  

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