4Pi microscopy reveals an impaired three-dimensional mitochondrial network of pancreatic islet beta-cells, an experimental model of type-2 diabetes

0349433 - FGÚ 2011 RIV NL eng J - Journal Article
Dlasková, Andrea - Špaček, Tomáš - Šantorová, Jitka - Plecitá-Hlavatá, Lydie - Berková, Z. - Saudek, F. - Lessard, M. - Bewersdorf, J. - Ježek, Petr
4Pi microscopy reveals an impaired three-dimensional mitochondrial network of pancreatic islet beta-cells, an experimental model of type-2 diabetes.
Biochimica Et Biophysica Acta-Bioenergetics. Roč. 1797, 6-7 (2010), s. 1327-1341. ISSN 0005-2728. E-ISSN 1879-2650
R&D Projects: GA MZd(CZ) NR9183; GA AV ČR(CZ) IAA500110701; GA MŠMT(CZ) ME09029
Institutional research plan: CEZ:AV0Z50110509
Keywords : 4Pi * diabetes * mitochondrial network morphology
Subject RIV: ED - Physiology
Impact factor: 5.132, year: 2010

Pathology of diabetic beta-cells might be reflected by the altered morphology of mitochondrial network. Its characterization is however hampered by the complexity and density of three-dimensional (3D) mitochondrial tubular network in these cell types. Mitochondrial network morphology in fixed cells can be studied by 4Pi microscopy, a laser scanning microscopy technique which provides an 7-fold improved axial resolution (100 nm) over conventional confocal microscopy. We demonstrate that beta-cells within the Langerhans islets from diabetic Goto Kakizaki rats exhibited a more disintegrated mitochondrial network compared to those from control Wistar rats and model insulinoma INS-1E cells
Permanent Link: http://hdl.handle.net/11104/0189677