Isolation of proteins from ejaculated and capacitated sperms by various extraction approaches

0349094 - BTÚ 2011 US eng A - Abstract
Zigo, Michal - Jonáková, Věra - Postlerová, Pavla
Isolation of proteins from ejaculated and capacitated sperms by various extraction approaches.
International Journal of Andrology. WILEY-BLACKWELL. Roč. 33, Supplement 1 (2010), s. 68-68. ISSN 0105-6263.
[6th European Congress of Andrology. 29.09.2010-01.10.2010, Athens]
R&D Projects: GA ČR(CZ) GA303/09/1285; GA MŠMT(CZ) 1M06011; GA MZd NS10009-4/2008
Institutional research plan: CEZ:AV0Z50520701
Keywords : spermatozoa * protein extraction * 2D electrophoresis * zona pellucida * glycoproteins
Subject RIV: CE - Biochemistry

Recognition and the binding of spermatozoa to the zona pellucida (ZP) is a crucial step in the fertilization process. Despite longstanding research efforts to identify the sperm proteins that recognize ZP receptors, their precise determination still remains questionable in various mammalian species. Sperm proteins of ejaculated and in vitro capacitated boar sperms were isolated by agents as follows: Triton X-100, Triton X-114, acetic acid, SDS, n-octyl-β-D-glucopyranosid, extraction by freezing-thawing and characterized by 1D, 2D electrophoreses glycoprotein staining and substrate zymographic methods. Results have shown the quantitative and qualitative differences in 1D, 2D protein and glycoprotein profiles depending upon the isolation protocol. Differences were observed between protein profiles of ejaculated and capacitated sperms as well. Zymograms supported the prediction that using various isolation protocols gives various profiles of enzymatically active molecules.
Permanent Link: http://hdl.handle.net/11104/0189424