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Protonation states of the key active site residues and structural dynamics of the glmS riboswitch as revealed by molecular dynamics
- 1.0347709 - BFÚ 2011 RIV US eng J - Journal Article
Banáš, P. - Walter, N.G. - Šponer, Jiří - Otyepka, M.
Protonation states of the key active site residues and structural dynamics of the glmS riboswitch as revealed by molecular dynamics.
Journal of Physical Chemistry B. Roč. 114, č. 26 (2010), s. 8701-8712. ISSN 1520-6106. E-ISSN 1520-5207
R&D Projects: GA MŠMT(CZ) LC06030; GA ČR(CZ) GA203/09/1476; GA MŠMT(CZ) GD203/09/H046; GA AV ČR(CZ) IAA400040802; GA AV ČR(CZ) 1QS500040581
Grant - others:GA MŠk(CZ) LC512
Program: LC
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : riboswitch * ribozyme * RNA structure * molecular dynamics simulations
Subject RIV: BO - Biophysics
Impact factor: 3.603, year: 2010
The glmS catalytic riboswitch is part of the 5’-untranslated region of mRNAs encoding glucosamine-6-phosphate (GlcN6P) synthetase (glmS) in numerous Gram-positive bacteria. Binding of the cofactor GlcN6P induces site-specific self-cleavage of the RNA. However, detailed reaction mechanism as well as protonation state of glmS reactive form remains still elusive. To probe the dominant protonation states of key active site residues, we carried out explicit solvent molecular dynamic simulations involving various protonation states of three crucial active site moieties observed in the available crystal structures: (i) guanine G40 (following the T. tengcongensis numbering), (ii) the GlcN6P amino/ammonium group, and (iii) the GlcN6P phosphate moiety.
Permanent Link: http://hdl.handle.net/11104/0006029
Number of the records: 1