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Protein structure-sensitive electrocatalysis at dithiothreitol-modified electrodes
- 1.0347221 - BFÚ 2011 RIV US eng J - Journal Article
Ostatná, Veronika - Černocká, Hana - Paleček, Emil
Protein structure-sensitive electrocatalysis at dithiothreitol-modified electrodes.
Journal of the American Chemical Society. Roč. 132, č. 27 (2010), s. 9408-9413. ISSN 0002-7863. E-ISSN 1520-5126
R&D Projects: GA AV ČR(CZ) KJB100040901; GA ČR(CZ) GP202/07/P497; GA MŠMT(CZ) LC06035
Grant - others:GA AV ČR(CZ) KAN400310651
Program: KA
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : constant current chronopotentiometry * mercury and solid amalgam electrodes * structure-sensitive protein analysis
Subject RIV: BO - Biophysics
Impact factor: 9.019, year: 2010
Dithiothreitol (DTT)-mercury and DTT-solid amalgam electrodes are proposed for protein microanalysis by means of constant current chronopotentiometric stripping (CPS). Self-assembled monolayers (SAMs) of DTT at the electrode surface protected surface-attached proteins from the electric field-driven denaturation. Using CPS peak H, native and denatured forms of bovine serum albumin and of other proteins were easily distinguished.
Permanent Link: http://hdl.handle.net/11104/0188047
Number of the records: 1