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The Activity of Ubiquitin Activating Enzyme UBA1 is Required for Sperm Capacitation, Acrosomal Exocytosis and Sperm-Egg Coat Penetration during Porcine Fertilization
- 1.0347158 - BTÚ 2011 US eng A - Abstract
Yi, Y.-J. - Zimmerman, S.W. - Manandhar, G. - Odhiambo, J.F. - Jonáková, Věra - Sutovsky, M. - Park, C.-S. - Sutovsky, P.
The Activity of Ubiquitin Activating Enzyme UBA1 is Required for Sperm Capacitation, Acrosomal Exocytosis and Sperm-Egg Coat Penetration during Porcine Fertilization.
Biology of Reproduction 2010 Supplement. Milwaukee: Society for the Study of Reproduction, 2010. s. 125. ISSN 0006-3363. E-ISSN 1529-7268.
[43rd Annual Meeting of the Society for the Study of Reproduction / "The Intersection between Genetics, Genomics, and Reproductive Biology"/. 30.07.2010-03.08.2010, Milwaukee]
Institutional research plan: CEZ:AV0Z50520701
Keywords : Ubiquitin * Acrosin-inhibitor * Spermadhesin * Sperm capacitation * Fertilization * Acrosomal exocytosis
Subject RIV: CE - Biochemistry
Ubiquitin binds covalently to substrate proteins through a multi-step enzymatic pathway, catalyzed by the enzymatic activities of the E1-type ubiquitin-activating enzyme (UBA1), ubiquitin-conjugating enzymes (UBE2), and E3-type ubiquitin ligase. In Western blotting with anti-ubiquitin antibodies, unusually high Mr bands, suggestive of posttranslational modification by ubiquitination, were detected in non-capacitated spermatozoa by Western blotting with antibodies specific to acrosomal proteins SPINK2 (acrosin-inhibitor) and AQN1 (spermadhesin). Thiol ester assays utilizing biotinylated substrate ubiquitin and isolated sperm acrosome fractions confirmed the enzymatic activity of UBA1 in the sperm acrosome, which was sensitive to UBA1-inhibitor. To our knowledge, this is the first report of UBA1-requirement for mammalian fertilization. It appears that UBA1 has an essential role in sperm capacitation and acrosomal function during fertilization.
Permanent Link: http://hdl.handle.net/11104/0187998
Number of the records: 1