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Method for resolution and quantification of components of the non-photochemical quenching (qN)
- 1.0346771 - BC 2011 RIV NL eng J - Journal Article
Roháček, Karel
Method for resolution and quantification of components of the non-photochemical quenching (qN).
Photosynthesis Research. Roč. 105, č. 2 (2010), s. 101-113. ISSN 0166-8595. E-ISSN 1573-5079
R&D Projects: GA AV ČR IAA600960716
Institutional research plan: CEZ:AV0Z50510513
Keywords : Dark relaxation * Fluorescence quenching * Multi-exponential regression
Subject RIV: BO - Biophysics
Impact factor: 2.410, year: 2010
A new method of the chlorophyll (Chl) fluorescence quenching analysis is described, which allows the calculation of values of (at least) three components of the non-photochemical quenching of the variable Chl a fluorescence (qN) using a non-linear regression of a multi-exponential function within experimental data. Formulae for coefficients of the “energy”-dependent quenching (qE), the state-transition quenching (qT) and the photo/inhibitory quenching (qI) of Chl a fluorescence were found on the basis of three assumptions: (i) the dark relaxation kinetics of qN, as well as of all its components, is of an exponential nature, (ii) the superposition principle is valid for individual Chl a fluorescence quenching processes, and (iii) the same reference fluorescence level (Fv) is used to define both qN and its components. All definitions as well as the algorithms for analytical recognition of the qN components are theoretically clarified and experimentally tested.
Permanent Link: http://hdl.handle.net/11104/0187703
Number of the records: 1