The assembly of F1FO-ATP synthase is disrupted upon interference of RNA editing in Trypanosoma brucei

0342884 - BC 2011 RIV AU eng J - Journal Article
Hashimi, Hassan - Benkovičová, V. - Čermáková, P. - Lai, De Hua - Horváth, A. - Lukeš, Julius
The assembly of F1FO-ATP synthase is disrupted upon interference of RNA editing in Trypanosoma brucei.
International Journal for Parasitology. Roč. 40, č. 1 (2010), s. 45-54. ISSN 0020-7519. E-ISSN 1879-0135
R&D Projects: GA ČR GA204/06/1558; GA AV ČR IAA500960705
Institutional research plan: CEZ:AV0Z60220518
Keywords : RNA editing * ATP synthase * mitochondrion * Trypanosoma * respiratory complex * membrane potential
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.822, year: 2010

Taking advantage of the different phenotypes between RNA interference knock-down cell lines in which the expression of proteins involved in mtRNA metabolism and editing can be silenced, we provide support for the view that ATP6 is encoded in the mt genome of Trypanosoma species and that it is incorporated into complex V. The reduction of the F1FO oligomer of complex V coincides with the accumulation of the F, moiety in ATP6-lacking cells, which also appear to lack the F-O ATP9 multimeric ring. The oligomycin sensitivity of ATPase activity of complex V in ATP6-lacking cells is reduced, reflecting the insensitivity of the Dk and Ak cells to this drug. In addition, the F, moiety of complex V appears to exist as a dimer in steady state conditions and contains the ATP4 subunit traditionally assigned to the F-O sub-complex.
Permanent Link: http://hdl.handle.net/11104/0185494