Rhodococci: Powerful bacterial degraders of aromatic compounds in environment

0340991 - MBÚ 2010 RIV CN eng A - Abstract
Szököl, Juraj - Knoppová, Monika - Pátek, Miroslav - Jirků, V.
Rhodococci: Powerful bacterial degraders of aromatic compounds in environment.
IUBMB /21./ and FAOBMB /12./ International Congress of Biochemistry and Molecular Biology. Shanghai: Chinese Society for Cell Biology, 2009. s. 58-58.
[IUBMB /21./ and FAOBMB /12./ International Congress of Biochemistry and Molecular Biology. 02.08.2009-07.08.2009, Shanghai]
R&D Projects: GA MŠMT 2B08062
Institutional research plan: CEZ:AV0Z50200510
Keywords : rhodococcus
Subject RIV: EE - Microbiology, Virology

Phenol-degrading strains Rhodococcus erythropolis CCM2595 and Rhodococcus jostii RHA1 were chosen for genetic analysis. We have cloned and sequenced the gene cluster (7 kb) responsible for phenol degradation in R. erythropolis strain: pheA1, pheA2 (subunits of phenol hydroxylase), catA (catechol 1,2-dioxygenase), catB (muconate cycloisomerase), catC (muconolactone isomerase), pheR (AraC-type regulator) and catR (IclR-type regulator). Genome sequence analysis of R. jostii RHA1 revealed presence of two similar clusters with the same arrangement of the phenol degradation genes. Using MALDI-TOF mass spectrometry, binding of the CRP-like protein upstream of the pheA2A1 genes of two Rhodococcus strains was proved. The CRP protein may be involved in regulation of substrate utilization hierarchy
Permanent Link: http://hdl.handle.net/11104/0184112