Genetic Control of Biosynthesis of Secondary Metabolites – Lincosamide Antibiotics as Examples

0340932 - MBÚ 2010 RIV KR eng A - Abstract
Spížek, Jaroslav - Novotná, Jitka - Olšovská, Jana - Smutná, Yvona - Kadlčík, Stanislav - Koběrská, Markéta - Ulanová, Dana - Najmanová, Lucie - Janata, Jiří
Genetic Control of Biosynthesis of Secondary Metabolites – Lincosamide Antibiotics as Examples.
Annual World Congress of Industrial Biotechnology 2009 /2./. Seoul: COEX Convention and Exhibition Center, 2009. s. 71-71.
[Annual World Congress of Industrial Biotechnology 2009 /2./. 05.04.2009-07.04.2009, Seoul]
R&D Projects: GA AV ČR IAA500200810
Institutional research plan: CEZ:AV0Z50200510
Keywords : lincomycin
Subject RIV: EE - Microbiology, Virology

Lincomycin and its derivatives are potent antibiotics used in human and veterinary medicine. Lincomycin molecule is composed of two subunits, methylthiolincosamide (MTL) and methylproline linked by an amide bond. The gene cluster of Streptomyces lincolnensis controlling lincomycin biosynthesis contains 29 ORFs with putative biosynthetic/regulatory (lmb) and resistance (lmr) functions. Six ORFs (lmbA, B1, B2, W, X, Y) were found to have their counterparts among genes controlling biosynthesis of anthramycin sharing with lincomycin the initial reactions of biosynthesis of proline derivatives. Functions of LmbB1 and LmbB2 proteins were demonstrated in vitro; by gene inactivation analysis lmbA, X, W genes were confirmed to be included in propylproline biosynthesis
Permanent Link: http://hdl.handle.net/11104/0184072