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Activity of the phenol catabolic enzymes from Rhodococcus erythropolis
- 1.0340755 - MBÚ 2010 RIV CH eng A - Abstract
Knoppová, Monika - Szököl, Juraj - Halada, Petr - Nešvera, Jan - Čejková, A. - Masák, J. - Jirků, V. - Pátek, Miroslav
Activity of the phenol catabolic enzymes from Rhodococcus erythropolis.
Biotrans 2009. Bern: Springer, 2009. s. 23-23.
[International Symposium on Biocatalysis /9./. 05.07.2009-09.07.2009, Bern]
R&D Projects: GA MŠMT 2B08062
Institutional research plan: CEZ:AV0Z50200510
Keywords : phenol
Subject RIV: EE - Microbiology, Virology
Induction of the phenol hydroxylase and catechol dioxygenase activity with phenol was suppressed by succinate in the growth medium, whereas presence of glucose did not influence it. The cluster of genes responsible for phenol degradation, pheA2A1RcatRABC, was cloned and sequenced. To improve the phenol-catabolic activity, recombinant strains R. erythropolis (pSRKcatRABC), R. erythropolis (pSRKpheA2A1R) and R. erythropolis catR were constructed. In all cases, the constructed strains degraded phenol faster than the WT-strain
Permanent Link: http://hdl.handle.net/11104/0183934
Number of the records: 1