Cancer cells with high expression of CD133 exert FLIP upregulation and resistance to TRAIL-induced apoptosis

0340567 - BTÚ 2010 RIV NL eng J - Journal Article
Zobalová, Renata - Stantic, M. - Prokopová, Kateřina - Dong, L. F. - Neužil, Jiří
Cancer cells with high expression of CD133 exert FLIP upregulation and resistance to TRAIL-induced apoptosis.
Biofactors. Roč. 34, č. 3 (2009), s. 231-235. ISSN 0951-6433. E-ISSN 1872-8081
R&D Projects: GA AV ČR(CZ) IAA500520702; GA ČR GA204/08/0811
Institutional research plan: CEZ:AV0Z50520701
Keywords : Cancer stem cells * CD 133 * FLIP
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.912, year: 2009

It is increasingly accepted that cancer stem cells (CSCs) are rather resistant to apoptosis to various inducers, including the immunological apoptogen TRAIL. Here we show that cancer cells with high expression of CD 133, a marker that is often associated with CSCs, are resistant to TRAIL-induced apoptosis, compared to their CD133-low counterparts. We show that this resistance can be ascribed to the high expression of FLIP, an inhibitor of the extrinsic apoptotic pathway, in CD133-high cells. Down¬regulation of FLIP by siRNA in CD133-high cells sensitised them to TRAIL killing. Thus, CD133-high cells may be resistant to TRAIL due to high expression of FLIP.
Permanent Link: http://hdl.handle.net/11104/0183780