Antitumor Effects and Cytotoxicity of Recombinant Plant Nucleases

0340098 - BC 2010 RIV US eng J - Journal Article
Matoušek, Jaroslav - Podzimek, Tomáš - Pouckova, P. - Stehlík, Jan - Škvor, J. - Souček, J. - Matoušek, Josef
Antitumor Effects and Cytotoxicity of Recombinant Plant Nucleases.
Oncology Research. Roč. 18, č. 4 (2009), s. 163-171. ISSN 0965-0407. E-ISSN 1555-3906
R&D Projects: GA ČR GA521/09/1214
Institutional research plan: CEZ:AV0Z50510513; CEZ:AV0Z50450515
Keywords : Anticarcinogenic and antiproliferative nucleases * Human melanoma * Tumor xenografts * Nicotiana benthamina
Subject RIV: FD - Oncology ; Hematology
Impact factor: 1.478, year: 2009

Antitumor effects and cytotoxicity of recombinant plant nucleases R-TBN1 and R-HBN1 were studied. The nucleases were strongly cytostatic in vivo after their administration intravenously as stabilized conjugates with polyethyleneglycol. Both nucleases were as effective against human melanoma tumors grown on mouse model as previously studied pine pollen (PN) and mung bean nucleases and their effects were reached at about 10 times lower concentrations compared to bovine seminal RNase (BS-RNase). Because R-HBN1 and R-TBN1 share only 67.4% amino acid identity and only partial immunochemical cross-reactivity, their similar anticancerogenic effects can be explained by their catalytical similarity. Both recombinant nucleases showed lower degree of aspermatogenesis compared to BS-RNase and PN nuclease. Owing to low cytotoxicity on the one hand, and high efficiency on the other, R-HBN1 and R-TBN1 appear to be stable biochemical agents that can be targeted as potential antitumor cytostatics.
Permanent Link: http://hdl.handle.net/11104/0183417