Practical Methods for Biocatalysis and Biotransformations

0339610 - MBÚ 2010 RIV GB eng M - Monography Chapter
Křen, Vladimír - Bojarová, Pavla
Glycosyl Azides: Novel Substrates for Enzymatic Transglycosylations.
Practical Methods for Biocatalysis and Biotransformations. Chippenham, Wiltshire: John Wiley and Sons Ltd, 2009 - (Whittall, J.; Sutton, P.), s. 232-235. ISBN 978-0-470-51927-1
Institutional research plan: CEZ:AV0Z50200510
Keywords : carbohydrate synthesis * glycosyl donor * glycosidase
Subject RIV: CE - Biochemistry

Enzymatic transglycosylation catalysed by glycosidases is a respected method in carbohydrate synthesis. The spectrum of acceptors is practically infinite, contrary to glycosyl donors, usually nitrophenyl glycosides. We have developed glycosyl azides as novel, efficient and easily prepared glycosyl donors for glycosidases. Their high water solubility facilitates the synthesis of disaccharides by transglycosylations with comparable or better yields than using traditional O-glycosides. Using the azide moiety, such products can simply be conjugated to complex structures after reduction of the azide to an amine
Permanent Link: http://hdl.handle.net/11104/0183083