Glucose determination using a re-usable enzyme-modified ion track membrane sensor

0338731 - ÚJF 2010 RIV GB eng J - Journal Article
Fink, Dietmar - Klinkovich, I. - Bukelman, O. - Marks, R.S. - Kiv, A. - Fuks, D. - Fahrner, W. R. - Alfonta, L.
Glucose determination using a re-usable enzyme-modified ion track membrane sensor.
Biosensors and Bioelectronics. Roč. 24, č. 8 (2009), s. 2702-2706. ISSN 0956-5663. E-ISSN 1873-4235
Institutional research plan: CEZ:AV0Z10480505
Keywords : Glucose sensor * etched tracks * Ion track membranes
Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders
Impact factor: 5.429, year: 2009

A novel concept for a glucose biosensor based on the enzyme glucose oxidase covalently linked to nanopores of etched nuclear track membranes is presented. This device can be used to detect physiologically relevant glucose concentrations between 10 mu M and 1 M. The sensitive catalytic sensor can be made re-usable due to the production of diffusible products from the oxidative biomolecular recognition event. We have demonstrated both the simplicity with which this sensor can be produced and the stability of the enzyme embedded in the nanopores. We strongly believe that the approach could be expanded for different enzymes and variable configurations of sensing and catalysis using membranes with nanopores for biocatalysis and enrichment of substrates and products. This is the first time that such devices are being used for biocatalytic transformations.
Permanent Link: http://hdl.handle.net/11104/0182428