The detection of the non-M2 muscarinic receptor subtype in the rat heart atria and ventricles

0338075 - FGÚ 2010 RIV DE eng J - Journal Article
Mysliveček, J. - Klein, M. - Nováková, M. - Říčný, Jan
The detection of the non-M2 muscarinic receptor subtype in the rat heart atria and ventricles.
[Detekce ne-M2 subtypu muskarinových recptorů v síních a komorách srdcí u potkanů.]
Naunyn-Schmiedeberg's Archives of Pharmacology. Roč. 378, č. 1 (2008), s. 103-116. ISSN 0028-1298. E-ISSN 1432-1912
R&D Projects: GA MŠMT(CZ) LC554; GA ČR(CZ) GA304/08/0256
Institutional research plan: CEZ:AV0Z50110509
Keywords : heart * muscarinic receptors * PLC activity
Subject RIV: FH - Neurology
Impact factor: 2.830, year: 2008

Our results show that only the M2 subtype is present in the atria. In the ventricles, however, we were able to determine that 20% (on average) of the muscarinic receptors were subtypes other than M2, with the majority of these belonging to the M1 subtype. We were also able to detect a marginal fraction (6±2%) of receptors that, based on other findings, belong mainly to the M5 muscarinic receptors. Co-treatment with MT7 and AQ-RA 741 was not a suitable tool for blocking of M1–M4 receptors and can not therefore be used as a method for M5 muscarinic receptor detection in substitution to crude venom. These results provide further evidence of the expression of the M1 muscarinic receptor subtype in the rat heart and also show that the heart contains at least one other, albeit minor, muscarinic receptor population, which most likely belongs to the M5 muscarinic receptors but not to that of the M3 receptors

V srdečních síních potkana jsme nalezli pouze M2 subtyp muskarinových receptorů, ale v komorách jsme naměřili asi 20% jiných subtypů než M2 (převážně M1 subtyp). Byli jsme také schopni detekovat malou frakci receptorů (6±2%) patřících pravděpodobně M5 muskarinovému podtypu. Naše výsledky poskytují další důkazy, že potkaní srdce obsahuje kromě M2 (majoritní) a M1 (minoritní) muskarinových receptorů také malou populaci dalšího, pravděpodobně M5 (ale ne M3) subtypu
Permanent Link: http://hdl.handle.net/11104/0181938