Post-transplant processing of superparamagnetic iron nanoparticles used for islet labeling and MR detection

0336523 - BC 2010 RIV DK eng A - Abstract
Zacharovová, K. - Berková, Z. - Herynek, V. - Girman, P. - Koblas, T. - Pektorová, L. - Mindlová, M. - Vávrová, E. - Vancová, Marie - Nebesářová, Jana - Dovolilová, E. - Bobek, V. - Saudek, F.
Post-transplant processing of superparamagnetic iron nanoparticles used for islet labeling and MR detection.
Xenotransplantation. Roč. 15, č. 5 (2009), s. 425-426. ISSN 0908-665X. E-ISSN 1399-3089.
[Joint Meeting of the International Pancreas and Islet Transplant Association / International Xenotransplantation Association. 12.10.2009-16.10.2009, Venice]
Institutional research plan: CEZ:AV0Z60220518
Keywords : nanoparticles * ferucarbotran
Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition

In vitro, ferucarbotran gradually incorporated into all types of islet cells and macrophages. First day after Tx, some particles were still in islet cell cytoplasm; however, many free iron particles were among the components released from the procedure-related destructed tissue. Components of dead cells including iron particles were engulfed by phagocytic cells. One week after Tx, ferucarbotran was rarely found in the endocrine cells, although the mechanism of iron release from beta-cells was not evident. Iron particles were stored in macrophages and in large fibroblast-resembling cells, which, however, were not positive for a fibroblast marker S100A4. One month after Tx, large amount of iron was concentrated in the islet surrounding macrophages and in the cells enclosed by collagen producing fibroblasts. No iron particles were found in beta cells. 3 months after Tx the iron particles were still detected.
Permanent Link: http://hdl.handle.net/11104/0180736