Identification and characterisation of lectins in several tick species

0336492 - BC 2010 RIV GB eng A - Abstract
Štěrba, J. - Dupejová, J. - Fišer, M. - Golovchenko, Maryna - Rudenko, Natalia - Grubhoffer, L.
Identification and characterisation of lectins in several tick species.
FEBS Journal. Roč. 276, S1 (2009), s. 88-88. ISSN 1742-464X. E-ISSN 1742-4658.
[34th FEBS Congress: Life's Molecular Interactions. 04.07.2009-09.07.2009, Prague]
R&D Projects: GA AV ČR KJB600960906; GA MŠMT(CZ) LC06009
Institutional research plan: CEZ:AV0Z60220518
Keywords : lectins * hemagglutination acitvity * fibrinogen- related domain
Subject RIV: CE - Biochemistry

FReD (fibrinogen-related domain) proteins are lectins with possible functions in immunity of invertebrates. Several FReD proteins were detected using antibodies directed against Dorin M, Ixodes ricinus hemolymph lectin, and hemagglutination acitivity of O. moubata and I. ricinus ticks. All identified FReD proteins are modified by truncated high-mannose glycans. Hemagglutination activity of hemolymph of the studied ticks was inhibited predominantly by N-acetylhexosamines and sialoglycoproteins. All tick species contained a hemelipoglycoprotein-like protein. Nucleotide sequence of novel protein containing fibrinogen- related domain was identified in ticks of species D. marginatus, Haemaphysalis punctata, and Hyalomma impeltatus. FReD proteins of D. marginatus localised in hemocytes, salivary glands, and gut cells.
Permanent Link: http://hdl.handle.net/11104/0180712