The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron

0332625 - BC 2010 RIV NL eng J - Journal Article
Pavingerová, Daniela
The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron.
Biologia Plantarum. Roč. 53, č. 4 (2009), s. 797-799. ISSN 0006-3134. E-ISSN 1573-8264
Institutional research plan: CEZ:AV0Z50510513
Keywords : plant growth regulators * tissue culture * Rhododendron
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.656, year: 2009

The influence of cytokinin thidiazuron (TDZ) and auxin indole-3-acetic acid (IAA) on in vitro shoot organogenesis of fifteen Rhododendron genotypes was investigated and a protocol for high frequency adventitious shoot regeneration from leaf explants was developed. High genotypic variation was observed and regeneration frequencies ranged from 0 to 100 %. Genotype Ovation had the highest number of shoots (26.4 per explant) after 12 weeks on medium with 0.57 uM IAA and 1.20 uM TDZ, but only 65 % of explants regenerated. Catawbiense Grandiflorum had 17.7 shoots per explant and 75 % regeneration on medium with 5.70 uM IAA and 0.45 uM TDZ and Van Werden Poelman had 14.3 shoots per explant and 100 % regeneration on medium with 0 57 uM IAA and 0.45 uM TDZ.
Permanent Link: http://hdl.handle.net/11104/0177832