Electrochemistry of nucleic acids and proteins. 3.-4. Electrochemistry of proteins and DNA-protein interactions

0330537 - BFÚ 2010 PL eng A - Abstract
Paleček, Emil
Electrochemistry of nucleic acids and proteins. 3.-4. Electrochemistry of proteins and DNA-protein interactions.
[Elektrochemie nukleových kyselin a bílkovin. 3.-4. Elektrochemie bílkovin a interakcí DNA-proteiny.]
Scientific Programme. Lańsk-Olsztyn, 2009. s. 1-2.
[III Summer School. 06.09.2009-18.09.2009, Lańsk-Olsztyn]
R&D Projects: GA ČR(CZ) GA301/07/0490; GA ČR(CZ) GP202/07/P497; GA MŠMT(CZ) LC06035
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : BSA denaturation * constant current chronopotentiometry of proteins * DNA-protein interactions
Subject RIV: BO - Biophysics

3. Using constant current chronopotentiometry with mercury electrodes electrocatalytic peak H can be obtained allowing stripping analysis of peptides and proteins at nanomolar concentrations. This procedure allowed detailed study of bovine serumalbumin denaturation and also pre-aggregation stages of alpha-synuclein aggregation. 4. The electrochemical study of DNA-protein interactions was reviewed.

3. Při chronopotenciometrii konstantním proudem na rtuťových elektrodách dovoluje peak H analýzu peptidů a bílkovin v nanomolárních koncentracích. Tento postup dovolil detailní studii denaturace hovězího serumalbuminu a také pre-agregačních stadií agregace alfa-synukleinu. 4. Byly shrnuty poznatky o elektrochemickém sledování interakcí DNA s proteiny.
Permanent Link: http://hdl.handle.net/11104/0176309