Metagenomics reveals diversity and abundance of meta-cleavage pathways in microbial communities from soil highly contaminated with jet fuel under air-sparging bioremediation

0330507 - MBÚ 2010 RIV GB eng J - Journal Article
Brennerová, Mária - Josefiová, Jiřina - Brenner, Vladimír - Pieper, D. H. - Junca, H.
Metagenomics reveals diversity and abundance of meta-cleavage pathways in microbial communities from soil highly contaminated with jet fuel under air-sparging bioremediation.
[Metagenomika odhaluje diverzitu a zastoupení meta-štěpících drah v mikrobiálních populacích z půd vysoce kontaminovaných leteckým olejem za air-spargingové bioremediace.]
Environmental Microbiology. Roč. 11, č. 9 (2009), s. 2216-2227. ISSN 1462-2912. E-ISSN 1462-2920
R&D Projects: GA MŠMT 1M06011; GA MŠMT 2B06156
Institutional research plan: CEZ:AV0Z50200510
Keywords : DIOXYGENASE GENE DIVERSITY * CATECHOL 2,3-DIOXYGENASE * AROMATIC-COMPOUNDS
Subject RIV: EE - Microbiology, Virology
Impact factor: 4.909, year: 2009

The extradiol dioxygenase diversity of a site highly contaminated with aliphatic and aromatic hydrocarbons under air-sparging treatment was assessed by functional screening of a fosmid library in Escherichia coli with catechol as substrate. The 235 positive clones from inserts of DNA extracted from contaminated soil were equivalent to one extradiol dioxygenaseencoding gene per 3.6 Mb of DNAscreened, indicating a strong selection for genes encoding this function

Diverzita genů pro extradiol dioxygenázy v půdách vysoce kontaminovaných leteckým petrolejem a za air-spargingové remediace byla určena funkčním screeningem fosmidní metagenomické knihovny použitím catecholu jako substrátu
Permanent Link: http://hdl.handle.net/11104/0176284