Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling

0330133 - ÚIACH 2010 GB eng A - Abstract
Černý, M. - Dyčka, Filip - Bobálová, Janette - Brzobohatý, B.
Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling.
[Změny v proteomu a fosfoproteomu v brzké odpovědi na cytokininy v Arabidopsis thaliana.]
FEBS Journal. Roč. 276, s1 (2009), s. 231. ISSN 1742-464X. E-ISSN 1742-4658.
[FEBS Congress /34./. 04.07.2009-09.07.2009, Prague]
R&D Projects: GA MŠMT 1M06030; GA AV ČR IAA600040701
Institutional research plan: CEZ:AV0Z40310501
Keywords : proteomic analysis * phosphoproteome * cytokinin
Subject RIV: CB - Analytical Chemistry, Separation
http://www3.interscience.wiley.com/cgi-bin/fulltext/122457210/PDFSTART

We employed 2-DE followed by image analysis and MALDI-TOF-TOF MS to analyze early changes in steady-state protein levels and phosphorylation status of the proteome in cytokinin treated Arabidopsis seedlings. Effects of four principal cytokinins (trans-zeatin, benzylaminopurine, thidiazuron and 6-(γ,γ-Dimethylallylamino)purine) were compared.

V této práci byly sledovány změny proteinového a fosfoproteinového profilu rostliny Arabidopsis v odpovědi na cytokininy pomocí metod 2-DE, analýzou obrazu a MALDI-TOF-TOF MS.
Permanent Link: http://hdl.handle.net/11104/0175990