Digestive alpha-amylases of the flour moth Ephestia kuehniella - adaptation to alkaline environment and plant inhibitors

0329444 - ÚOCHB 2010 GB eng A - Abstract
Pytelková, Jana - Hubert, J. - Lepšík, Martin - Šobotník, Jan - Šindelka, Radek - Křížková, I. - Horn, Martin - Mareš, Michael
Digestive alpha-amylases of the flour moth Ephestia kuehniella - adaptation to alkaline environment and plant inhibitors.
FEBS Journal. Roč. 276, Suppl. 1 (2009), s. 162-162. ISSN 1742-464X. E-ISSN 1742-4658.
[FEBS Congress /34/. 04.07.2009-09.07.2009, Praha]
R&D Projects: GA ČR GP525/09/P600; GA AV ČR IAA400550617
Institutional research plan: CEZ:AV0Z40550506; CEZ:AV0Z50520701
Keywords : Ephestia kuehniella * alpha amylase * alkaline adaptation
Subject RIV: CE - Biochemistry

Alkaline digestive α-amylases were investigated in the flour moth Ephestia kuehniella, an important stored-product pest. The cDNA sequence was determined, and a homology model was built in order to analyze structural features responsible for adaptation to alkaline pH. Interaction of these enzymes with alpha-amylase inhibitors of plant origin was characterized in vitro and in vivo.
Permanent Link: http://hdl.handle.net/11104/0175479