Quest for protein biomarkers of neural stem cells differentiation

0329309 - ÚŽFG 2010 RIV CZ eng K - Conference Paper (Czech conference)
Skalníková, Helena - Halada, Petr - Vodička, Petr - Motlík, Jan - Horing, O. - Jensen, O. N. - Gadher, S. J. - Pelech, S. - Kovářová, Hana
Quest for protein biomarkers of neural stem cells differentiation.
1st Central and Eastern European Proteomic Conference and 3rd Czech Proteomic Conference. Mělník: ÚŽFG, 2007, s. 9-9.
[1st Central and Eastern European proteomic Conference and 3rd Czech Proteomic Conference. Praha (CZ), 29.10.2007-31.10.2007]
R&D Projects: GA MŠMT LC545; GA MŠMT 1M0538
Institutional research plan: CEZ:AV0Z50450515; CEZ:AV0Z50200510
Keywords : neurogenesis * cell diferentiation
Subject RIV: FH - Neurology

Understanding neurogenesis and neural cell differentiation presents a unique challenge for treatment of nervous system disorders. To gain more insight about molecular mechanism of differentiation of neural cells, we applied different proteomic approaches using classical 2-DE followed by MS and antibody microarrays. Based on 2-DE, profile of constituent proteins of neural stem cells and their differentiated progenies was estabilished at first and then the protein species that are significantly up or down regulated during the differentiation were selected. Differentiation of neural cells was accompanied by changes in the expression of proteins involved in DNA and RNA binding, mRNA processing and transport, stress responses, iron storage and redox regulation. Immunoblot verified changes of hnRNP A1, hnRNP A2/B1, RafB, heme-oxygenasy 2, GRK2 proteins and alphaB-crystallin (S45), CDK1/2 (Y15), PKC mu (S738+S742), proline-rich Akt substrate (T246) phosphorylations during differentiation.
Permanent Link: http://hdl.handle.net/11104/0175381