Localization of porcine beta-microseminoprotein in reproductive organs and spermatozoa

0326013 - BTÚ 2010 CZ eng A - Abstract
Postlerová, Pavla - Davidová, Nina - Jonáková, Věra
Localization of porcine beta-microseminoprotein in reproductive organs and spermatozoa.
Sborník - XV. Symposium českých reprodukčních imunologů s mezinárodní účastí. Praha: BTO-N, 2009. s. 33-33.
[XV. Symposium českých reprodukčních imunologů s mezinárodní účastí. 29.05.2009-31.05.2009, Žďár nad Sázavou]
R&D Projects: GA MŠMT(CZ) 1M06011; GA ČR GD523/08/H064; GA ČR GA303/09/1285
Institutional research plan: CEZ:AV0Z50520701
Keywords : porcine beta-microseminoprotein * male reproductive organs * spermatozoa
Subject RIV: EC - Immunology

Beta-microseminoprotein (MSP) is a predominat protein of human seminal plasma of the prostate origin. Origin and localization of porcine MSP in reproductive tract are unresolved. We proved mRNA transcripts of MSP gene in all reproductive organs. These results were supported by reaction with the specific MSP antibody in fluids, extracts and secretory tissues of reproductive organs. Immunofluorescence displayed the MSP on epididymal and ejaculated sperm in the acrosomal region. The MSP absence on the surface of capacitated sperm together with the antibody detection in the sperm acidic extract after capacitation indicated its presence in the acrosomal content. Additionally, MSP localization in the sperm acrosome was shown by immunofluorescence in capacitated sperm with permeabilized membrane. The MSP function in seminal plasma and spermatozoa is not fully understood. Nevertheless, the MSP secretion by different reproductive organs might indicate its multiple roles in reproductive process.
Permanent Link: http://hdl.handle.net/11104/0173256