0325896 - ÚEB 2009 RIV GB eng J - Journal Article
Lewis, S. - Faricelli, M. E. - Appendino, M. L. - Valárik, Miroslav - Dubcovsky, J.
The chromosome region including the earliness per se locus Eps-A(m)1 affects the duration of early developmental phases and spikelet number in diploid wheat.
[The chromosome region including the earliness per se locus Eps-A(m)1 affects the duration of early developmental phases and spikelet number in diploid wheat.]
Journal of Experimental Botany. Roč. 59, č. 13 (2008), s. 3595-3607. ISSN 0022-0957. E-ISSN 1460-2431
Institutional research plan: CEZ:AV0Z50380511
Keywords : Development * earliness per se * heading time
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.001, year: 2008

Earliness per se genes are those that regulate flowering time independently of vernalization and photoperiod, and are important for the. ne tuning of flowering time and for the wide adaptation of wheat to different environments. The earliness per se locus Eps-A(m)1 was recently mapped within a 0.8 cM interval on chromosome 1A(m)L of diploid wheat Triticum monococcum L., and it was shown that its effect was modulated by temperature. In this study, this precise mapping information was used to characterize the effect of the Eps-A(m)1 region on both duration of different developmental phases and spikelet number. Near isogenic lines (NILs) carrying the Eps-A(m)1-l allele from the cultivated accession DV92 had significantly longer vegetative and spike development phases (P < 0.0001) than NILs carrying the Eps-A(m)1-e allele from the wild accession G3116. These differences were paralleled by a significant increase in the number of spikelets per spike, in both greenhouse and field experiments (P < 0.0001). Significant interactions between temperature and Eps-A(m)1 alleles were detected for heading time (P < 0.0001) but not for spikelet number (P = 0.67). Experiments using NILs homozygous for chromosomes with recombination events within the 0.8 cM Eps-A(m)1 region showed that the differences in number of spikelets per spike were linked to the differences in heading time controlled by the Eps-A(m)1 locus. These results indicate that the differences in these two traits are either pleiotropic effects of a single gene or the effect of closely linked genes. A similar effect on spikelet number was detected in the distal region of chromosome 1AL in common wheat (T. aestivum L.).

Earliness per se geny regulují kvetení pšenice nezávisle na jarovizaci a jsou zodpovědné za jemnou regulaci kvetení a přizpůsobování pšenice širokému spektru přírodních podmínek. Earliness per se lokus Eps-Am1 byl nedávno mapovaný v intervalu 0,8 cM na chromozómu Am1 diploidní pšenice T. monococcum L. a jeho efekt je ovlivňován teplotou. V této studii byl charakterizován účinek Eps-Am1 regionu na trvání raných vývojových fází a počtu obilek u pšenice. Téměř izogenní linie nesoucí Eps-Am1 alelu z domestikovaného kultivaru DV92 (se sledovaným významným zvýšením počtu obilek na klas) měly významně delší vegetativní a generativní fáze než linie s alelou z planého kultivaru G3116. Byla pozorována statisticky významná interakce mezi teplotou a dobou od zasazení po kvetení, kontrolovaná Eps-Am1 lokusem, interakce mezi teplotou a počtem obilek na klas pozorována nebyla. Tyto výsledky indikují, že tyto dvě vlastnosti jsou buď pleiotropním efektem jednoho genu nebo účinky genů v těsné vazbě.
Permanent Link: http://hdl.handle.net/11104/0173177