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Hybrid surface platform for the simultaneous detection of proteins and DNA using a surface plasmon resonance (SPR) imaging sensor
- 1.0325818 - ÚFE 2009 RIV US eng J - Journal Article
Homola, Jiří - Piliarik, Marek - Ladd, J. - Taylor, A. - Shaoyi, J.
Hybrid surface platform for the simultaneous detection of proteins and DNA using a surface plasmon resonance (SPR) imaging sensor.
[Měřící platforma využívající hybridní povrchy pro souběžnou detekci proteinu a DNA pomocí SPR zobrazování.]
Analytical Chemistry. Roč. 80, č. 11 (2008), s. 4231-4236. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA AV ČR KAN200670701
Institutional research plan: CEZ:AV0Z20670512
Keywords : Surface plasmon resonance imaging * DNA-directed immobilization * protein array
Subject RIV: JB - Sensors, Measurment, Regulation
Impact factor: 5.712, year: 2008
In this work, we present a novel surface and assay for the simultaneous detection of DNA and protein analytes on a surface plasmon resonance (SPR) imaging sensor. DNA sequences are spotted onto a gold-coated glass substrate. Antibodies conjugated with complementary single-stranded DNA sequences are immobilized on the surface through DNA hybridization.
Byla vyvinuta nová imobilizační metoda pro souběžnou detekci DNA a proteinů. DNA sekvence byly naspotovány na pozlacené substráty. protilátky byly konjugovány s komplementárnímy DNA sekvencemi a immobilizovány na povrch skrze DNA hybridizaci.
Permanent Link: http://hdl.handle.net/11104/0173112
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