Susceptibility of some clinical isolates of Staphylococcus aureus to fractions from the aerial parts of Leuzea carthamoides

0323124 - ÚEB 2009 RIV PL eng J - Journal Article
Janovská, D. - Klouček, P. - Urban, J. - Vaněk, Tomáš - Rada, V. - Kokoška, L.
Susceptibility of some clinical isolates of Staphylococcus aureus to fractions from the aerial parts of Leuzea carthamoides.
[CITLIVOST NĚKTERÝCH KLINICKÝCH IZOLÁTŮ STAPHYLOCOCCUS AUREUS K FRAKCÍM Z NADZEMNÍCH ČÁSTÍ LEUZEA CARTHAMOIDES.]
Biologia. Roč. 63, č. 5 (2008), s. 607-609. ISSN 0006-3088. E-ISSN 1336-9563
R&D Projects: GA MŠMT 1P04OC926.001
Institutional research plan: CEZ:AV0Z50380511
Keywords : Leuzea carthamoides * aerial parts * antimicrobial activity
Subject RIV: CE - Biochemistry
Impact factor: 0.406, year: 2008

The antimicrobial activity of the dichloromethane extract from aerial parts of Leuzea carthamoides DC. was tested in vitro against 19 Staphylococcus aureus strains (ATCC 25923, CNCTC Mau 43/60, clinical isolates). The extract was fractionated by column chromatography on silica gel into six fractions (petroleum ether, toluene, dichloromethane, ethyl acetate, methanol and water). The minimum inhibitory concentrations (MICs) of the fractions ranged from 64 to 1024 mu g/mL. An ethyl acetate fraction (EA 1) with the widest range of activity inhibited all of the strains with MIC in the range 128-512 mu g/mL. This fraction exhibited potent activity against strains which showed associated resistance to oxacillin, ciprofloxacin and erythromycin.

Antimikrobiální aktivita dichlormetanového extraktu z nadzemních částí Leuzea carthamoides DC. byla testována in vitro proti 19 kmenům Staphylococcus aureus (ATCC 25923, CNCTC Mau 43/60, clinické izoláty). Extrakt byl frakcionován na kolonové chromatografii na silikagelu na šest frakcí (petrol ether, toluen, dichloromethan, ethyl acetat, methanol a voda).
Permanent Link: http://hdl.handle.net/11104/0171183