Real Time RT-PCR with a Newly Designed Set of Promers Confirmed the Presence of 2b and 2x/d Myosim Heavy Chain mRNAs in the Rat Slow Soleus Muscle

0320912 - FGÚ 2009 RIV CZ eng J - Journal Article
Žurmanová, Jitka - Půta, F. - Stopková, R. - Soukup, Tomáš
Real Time RT-PCR with a Newly Designed Set of Promers Confirmed the Presence of 2b and 2x/d Myosim Heavy Chain mRNAs in the Rat Slow Soleus Muscle.
[RT-PCR v reálném čase mRNA pro 2x/d a 2b izoformy myozinu v pomalém svalu m. soleus potkana.]
Physiological Research. Roč. 57, č. 6 (2008), s. 973-978. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA MŠMT(CZ) LC554; GA ČR(CZ) GA305/06/1115; GA ČR(CZ) GA304/08/0256
Grant - others:EC(XE) LSH-CT-2004-511978
Institutional research plan: CEZ:AV0Z50110509
Keywords : rat slow soleus muscle * myosin heavy chain isoforms * real time RT-PCR
Subject RIV: ED - Physiology
Impact factor: 1.653, year: 2008

The experiments were performed on 8-month-old inbred female Lewis strain rats used in our current study of regenerating transplanted muscles. The real time RT-PCR measurement confirmed the expression of all four MyHC mRNAs (type 1, 2a, 2x/d and 2b) in both fast extensor digitorum longus and slow soleus muscles, although in the soleus muscle of adult rats, only type 1 and 2a protein isoforms can be usually detected

Experimenty na potkanech starých 8 měsíců potvrdily, že i pomalý sval m. soleus exprimuje mRNA pro rychlé izoformy myozinu, podobně jako rychlé svaly, ačkoliv na úrovni proteinů je množství 2x/d a 2b izoforem na hladině detekce pomocí SDS_PAGE
Permanent Link: http://hdl.handle.net/11104/0169638