Properties of mixed cationic membranes studied by fluorescence solvent relaxation

0316906 - ÚFCH JH 2009 RIV US eng J - Journal Article
Olžyńska, Agnieszka - Jurkiewicz, Piotr - Hof, Martin
Properties of mixed cationic membranes studied by fluorescence solvent relaxation.
[Vlastnosti smíšených kationických membrán studované pomocí fluorescenční relaxace rozpouštědla.]
Journal of Fluorescence. Roč. 18, č. 5 (2008), s. 925-928. ISSN 1053-0509. E-ISSN 1573-4994
R&D Projects: GA AV ČR IAA400400503; GA MŠMT(CZ) LC06063
Institutional research plan: CEZ:AV0Z40400503
Keywords : time-resolved emission spectroscopy * solvent relaxation * membrane interface
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 1.880, year: 2008

The need for detailed biophysical description of cationic lipid membranes, which are commonly used as gene transfection vectors, led us to study the properties of mixed cationic/zwitterionic lipid bilayers. Fluorescence solvent relaxation measurements of 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan) incorporated in a membrane consisting of cationic dimyristoyltrimethylammoniumpropane (DMTAP) and zwitterionic dimyristoylphosphatidylcholine (DMPC) were performed. The obtained results are compared with a recently measured system consisting of dioleoyltrimethylammoniumpropane (DOTAP) and dioleoylphosphatidylcholine (DOPC) (Jurkiewicz et al. Langmuir 22:8741-8749, 2006). The similar nonmonotonic dependence of the relaxation kinetics on cationic lipid content in the membrane was present for both systems.

V této práci se studují vlastnosti smíšených kationických membrán studované pomocí fluorescenční relaxace rozpouštědla.
Permanent Link: http://hdl.handle.net/11104/0166686