Staphylococcus sp. strains isolated from the common vole - the isolates of a new staphylococcal species

0315061 - ÚBO 2009 TR eng A - Abstract
Nováková, D. - Hubálek, Zdeněk - Sedláček, I.
Staphylococcus sp. strains isolated from the common vole - the isolates of a new staphylococcal species.
XII. International Congress of Bacteriology and Applied Microbiology, 5-9 August 2008, Istanbul: Abstract Book. Istanbul: International Union of Microbiological Societies, 2008. s. 151.
[International Congress of Bacteriology and Applied Microbiology /12./. 05.08.2008-09.08.2008, Istanbul]
Institutional research plan: CEZ:AV0Z60930519
Keywords : Staphylococcus * common vole
Subject RIV: EE - Microbiology, Virology
http://www.iums2008.org/BAM_pdf.pdf

Two strains of Gram-positive cocci isolated from viscera of common voles (Microtus arvalis) were studied by phenotyping and genotyping. Biochemical features were tested by Api Staph kits (bioMérieux) and by conventional tests. Detailed characterization was done by ribotyping, whole-cell protein analysis and rep-PCR using (GTG)5 primer. The phylogenetic position was analysed by partial 16S rRNA sequencing. Both catalase positive, oxidase negative strains, resistant to bacitracin and sensitive to novobiocin and furazolidon were identified as Staphylococcus sp. Strains did not show unambiguous species identification based on phenotyping. Analysed strains revealed high mutual similarity of ribotype (EcoRI 88.9% and HindIII 78.6%), protein (95.6%) as well as rep-PCR (98.2%) fingerprints and concurrently were clearly distinct from all validly described staphylococcal taxa. The isolates showed the same partial 16S rRNA sequences, and the maximum sequence similarities were obtained with Staphylococcus muscae (98.3%) and Staphylococcus chromogenes (98.2%) type cultures - their sequences differed in 7 and 11 nucleotides, respectively. The obtained results proved that the tested isolates are two different strains representing probably a novel staphylococcal species. The study was partly supported by projects MSM0021622416 and AV0Z60960519.
Permanent Link: http://hdl.handle.net/11104/0165381