Bacterial communities of two contrasting soils reacted differently to lincomycin treatment

0314583 - MBÚ 2009 RIV NL eng J - Journal Article
Čermák, Ladislav - Kopecký, Jan - Novotná, Jitka - Omelka, M. - Parkhomenko, Natalia - Plháčková, Kamila - Ságová-Marečková, Markéta
Bacterial communities of two contrasting soils reacted differently to lincomycin treatment.
[Bakteriální společenstva dvou různých půd reagovala odlišně na opracovaní linkomycinem.]
Applied Soil Ecology. Roč. 40, č. 2 (2008), s. 348-358. ISSN 0929-1393. E-ISSN 1873-0272
R&D Projects: GA AV ČR IAA600200519; GA AV ČR IAA6020410; GA MŠMT 1M06011; GA MŠMT(CZ) LC06024
Grant - others:GA MZe(CZ) 0002700603
Institutional research plan: CEZ:AV0Z50200510
Keywords : bacterial diversity * forest soils * lincomycin
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.247, year: 2008

The antibiotic lincomycin an anthropogenic pollutant –may change the bacterial diversity and resistance of soil communities by increasing selection pressure in favour of resistant phenotypes. In a laboratory experiment, two forest soils of contrasting characteristics in both pH and clay content were examined for changes in their bacterial communities after lincomycin treatment. The two soils were incubated in beakers and treated with three dosages of lincomycin. Changes in bacterial community were assessed by (i) cultivation of bacteria on two different media, and percentage of resistant colonies at two lincomycin concentrations;(ii) analysis of putative actinomycete isolates for antibiotic activities and resistance; (iii) TRFLP diversity profiles of the 16S rDNA and homologues of the resistance gene lmrB; and (iv) cloning-sequencing of PCR amplicons of both 16S rDNA and lmrB homologues

Antropogenní polutant antibiotikum linkomycin může měnit bakteriální diversitu a rezistenci půdních společenstev zvýšením selekčního tlaku ve prospěch rezistentních fenotypů. Dvě lesní půdy s kontrastujícími pH a obsahem jílu byly testovány na změny v bakterialních komunitách po opracování linkomycinem
Permanent Link: http://hdl.handle.net/11104/0165048